Cell length measurements in longitudinal smooth muscle strips of the pig urinary bladder

Abstract

In this study the length of smooth muscle cells in muscle bundles of pig urinary bladder wall was determined after dissection in Tyrode buffers with different calcium concentrations ([Ca2+]). Previous studies have shown that the length of isolated smooth muscle cells decreases with an increase in [Ca2+] in the buffer. Unlike the results in isolated cells, no significant differences in length were found between cells in strips subjected to different [Ca2+]. Cells in bundles dissected from filled bladders were significantly larger than those dissected from emptied bladders. Cells in strips from emptied bladders dissected in 1.8 mM Ca(2+)-Tyrode buffer were shorter than those obtained in Ca(2+)-free buffer. From the measurements it was concluded that: (1) Cell length in intact tissue is directly related to tissue length; series elastic structures external to the cells do not allow significant shortening of the cells. (2) Passive parallel elasticity outside the cells accounts for passive shortening when bladders are emptied manually. (3) Cell length is not related to empty bladder weight. (4) A positive relation exists between empty bladder weight and bladder capacity.