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. 1993 Sep;59(9):3070-5.
doi: 10.1128/aem.59.9.3070-3075.1993.

Production of L-dihydroxyphenylalanine in Escherichia coli with the tyrosine phenol-lyase gene cloned from Erwinia herbicola

F Foor  1 , N Morin, K A Bostian
Affiliations

Production of L-dihydroxyphenylalanine in Escherichia coli with the tyrosine phenol-lyase gene cloned from Erwinia herbicola

F Foor et al. Appl Environ Microbiol. 1993 Sep.

Abstract

The gene (tutA) encoding tyrosine phenol-lyase from Erwinia herbicola was cloned into Escherichia coli, and fusions to the lac and tac promoters were constructed. The enzyme was expressed at high levels in E. coli in the presence of isopropyl-beta-D-thiogalactopyranoside or lactose as an inducer. L-Dihydroxyphenylalanine was synthesized in high yield from catechol, pyruvate, and ammonia by induced cells.

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References

    1. J Bacteriol. 1986 Jul;167(1):383-6 - PubMed
    1. Mol Microbiol. 1991 Jul;5(7):1585-92 - PubMed
    1. Genetics. 1987 Jun;116(2):191-9 - PubMed
    1. Prikl Biokhim Mikrobiol. 1985 Mar-Apr;21(2):161-6 - PubMed
    1. J Bacteriol. 1977 Nov;132(2):453-61 - PubMed

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