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Comparative Study
. 1993 Sep;59(9):3141-4.
doi: 10.1128/aem.59.9.3141-3144.1993.

Comparison and genomic sizing of Escherichia coli O157:H7 isolates by pulsed-field gel electrophoresis

K D Harsono  1 , C W Kaspar, J B Luchansky
Affiliations
Comparative Study

Comparison and genomic sizing of Escherichia coli O157:H7 isolates by pulsed-field gel electrophoresis

K D Harsono et al. Appl Environ Microbiol. 1993 Sep.

Abstract

Genomic DNAs of Escherichia coli O157:H7 strains isolated from patients and food samples were analyzed by pulsed-field gel electrophoresis. The rare-cutting endonucleases SfiI and XbaI generated 6 and 10 distinct genomic profiles, respectively, for the 22 strains analyzed, indicating that this technique may find application for epidemiologic studies. Summation of XbaI fragments from five E. coli O157:H7 strains estimated the genomic length at ca. 4.7 Mb.

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References

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