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. 1993 Sep;59(9):3150-3.
doi: 10.1128/aem.59.9.3150-3153.1993.

Molecular cloning and sequence analysis of the crtB gene of Thermus thermophilus HB27, an extreme thermophile producing carotenoid pigments

T Hoshino  1 , R Fujii, T Nakahara
Affiliations

Molecular cloning and sequence analysis of the crtB gene of Thermus thermophilus HB27, an extreme thermophile producing carotenoid pigments

T Hoshino et al. Appl Environ Microbiol. 1993 Sep.

Abstract

We have cloned and sequenced a 1.5-kb chromosomal fragment of Thermus thermophilus which promoted the overproduction of carotenoids in T. thermophilus. An open reading frame (ORF-A) coding for a polypeptide with 289 amino acids was responsible for carotenoid overproduction. The putative ORF-A protein showed significant homology with the amino acid sequences of crtB gene products (phytoene syntheses) of other microorganisms. The clone containing the ORF-A on a multicopy plasmid produced about three times as much carotenoid as that produced by the host strain, suggesting that the crtB gene product is a rate-limiting enzyme for carotenoid biosynthesis in T. thermophilus.

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References

    1. J Bacteriol. 1971 Oct;108(1):227-35 - PubMed
    1. J Bacteriol. 1972 May;110(2):691-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. J Gen Microbiol. 1978 Feb;104(2):193-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Feb;83(3):576-80 - PubMed

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