doi: 10.1006/bbrc.1993.2172.
The mechanism for apo-B mRNA editing is deamination
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- PMID: 8216250
- DOI: 10.1006/bbrc.1993.2172
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The mechanism for apo-B mRNA editing is deamination
Biochem Biophys Res Commun.
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Abstract
Apolipoprotein (apo-) B mRNA editing at nucleotide 6666 converts cytidine to uridine, transforming the codon for glutamine-2153 to a termination codon. To investigate this editing mechanism, [a-32P] and [5-3H] CTP were incorporated into synthetic apo-B RNA. After the substrate had been edited extensively in vitro by a partially purified editing extract, the edited base was isolated and analyzed for radioactivity. The uridine-6666 resulting from the editing reaction had the same ratio of 3H to 32P as did the cytidine-6666, demonstrating that deamination rather than base exchange or nucleotide replacement is the mechanism for apo-B mRNA editing.
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