[Isopycnic centrifugation in metrizanide gradient of ribosomal ribonucleoprotein precursor particles]
[Article in
French]
- PMID: 821661
Item in Clipboard
[Isopycnic centrifugation in metrizanide gradient of ribosomal ribonucleoprotein precursor particles]
[Article in
French]
C R Acad Hebd Seances Acad Sci D.
.
Abstract
Metrizamide has been described as a new medium for isopycnic centrifugation of unfixed biological particles. In the murine leukemia cell line L 5178 Y, the buoyant density of 80 S and 60 S preribosomal particles was found to be 1,22 g/cm3, which is noticeably lighter than buoyant densities of mature ribosomal particles.
Similar articles
-
Purification of messenger ribonucleoprotein particles from rabbit reticulocytes by zonal centrifugation in metrizamide.Eur J Biochem. 1981 Mar 16;115(1):45-52. doi: 10.1111/j.1432-1033.1981.tb06195.x. Eur J Biochem. 1981. PMID: 7227370
-
Size heterogeneity of the structural units of brain nuclear ribonucleoprotein particles.Mol Biol Rep. 1976 Apr;2(5):385-91. doi: 10.1007/BF00366260. Mol Biol Rep. 1976. PMID: 1272263
-
Messenger ribonucleoprotein particles from plant cell cultures.Z Naturforsch C Biosci. 1978 May-Jun;33(5-6):359-62. doi: 10.1515/znc-1978-5-609. Z Naturforsch C Biosci. 1978. PMID: 150147
-
The protein moieties of animal messenger ribonucleoproteins.FEBS Lett. 1973 Nov 15;37(1):1-6. doi: 10.1016/0014-5793(73)80413-2. FEBS Lett. 1973. PMID: 4127753 Review. No abstract available.
-
Fractionation of chromatin by buoyant density-gradient sedimentation in metrizamide.Methods Cell Biol. 1978;18:23-39. doi: 10.1016/s0091-679x(08)60130-2. Methods Cell Biol. 1978. PMID: 355790 Review. No abstract available.
Cited by
-
Metrizamide dissociates nuclear particles containing heterogeneous nuclear RNA.Nucleic Acids Res. 1977 Nov;4(11):3931-41. doi: 10.1093/nar/4.11.3931. Nucleic Acids Res. 1977. PMID: 593895 Free PMC article.