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. 1993 Jul 16;216(1-2):145-51.
doi: 10.1016/0009-8981(93)90147-v.

Erythrocyte galactokinase assay with high performance liquid chromatography

N Mizoguchi  1 , T Eguchi, N Sakura, K Ueda
Affiliations

Erythrocyte galactokinase assay with high performance liquid chromatography

N Mizoguchi et al. Clin Chim Acta. .

Abstract

An assay for erythtocyte galactokinase based on high performance liquid chromatographic determination of galactose 1-phosphate (Gal-1-P) is described. The determination of Gal-1-P was applied to a post-column fluorometric detection of reducing sugars using arginine. This method is as sensitive and accurate as conventional radioisotopic methods, but needs no radioisotopic facilities. It requires only a small blood sample and is suitable as a follow-up test in neonatal screening.

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