Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Dec;12(12):4593-601.
doi: 10.1002/j.1460-2075.1993.tb06148.x.

Developmental precise excision of Oxytricha trifallax telomere-bearing elements and formation of circles closed by a copy of the flanking target duplication

K Williams  1 , T G Doak, G Herrick
Affiliations

Developmental precise excision of Oxytricha trifallax telomere-bearing elements and formation of circles closed by a copy of the flanking target duplication

K Williams et al. EMBO J. 1993 Dec.

Abstract

The 4.1 kbp TBE1 elements of Oxytricha fallax and Oxytricha trifallax are deduced to transpose into a centrisymmetric target, CAnTG, and to duplicate the central AnT. Despite conserved C(A4C4)2 telomeric repeats at their tips, free TBE1s found during macronuclear development are not linear but 4.1 kbp circles closed on one copy of the AnT target duplication. The macronucleus-destined flanks are rejoined to regenerate the target, effecting efficient and precise somatic reversion of the germline transpositional mutation. A model is presented in which transposase catalyzes concerted precise rejoining of the flanks and cyclization of the excised element.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4992-6 - PubMed
    1. Trends Genet. 1992 Dec;8(12):439-45 - PubMed
    1. Nucleic Acids Res. 1983 Oct 25;11(20):7137-40 - PubMed
    1. Cell. 1984 Apr;36(4):1045-55 - PubMed
    1. Mol Cell Biol. 1984 May;4(5):931-8 - PubMed

Publication types

Associated data