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. 1976 Aug;73(8):2881-4.
doi: 10.1073/pnas.73.8.2881.

Lymphocyte stimulation: a rapid multiparameter analysis

Z Darzynkiewicz, F Traganos, T Sharpless, M R Melamed

Lymphocyte stimulation: a rapid multiparameter analysis

Z Darzynkiewicz et al. Proc Natl Acad Sci U S A. 1976 Aug.

Abstract

Several parameters of stimulation of individual lymphocytes are measured simultaneously by flow-cytofluorometry after differential staining of cellular DNA and RNA with the metachromatic fluorescent dye acridine orange. The method provides a means of analyzing the progression of stimulated cells through the cell cycle (G1, S and G2 + M), in addition to measuring the accumulation of RNA per cell; the RNA parameter is useful in distinguishing G1 from G0 cells. The multiparameter analysis may be of unique value in discriminating cases where the transcriptional and proliferative responses of lymphocytes to stimulants are not correlated.

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