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Comparative Study
. 1993 Aug;17(4):363-71.
doi: 10.3109/03630269308997489.

Two new rat alpha-globin sequences as identified by the conserved region PCR

V M Lam  1 , Y L Gu, D M Au, W M Wong, C W Ma, L Y Cheng
Affiliations
Comparative Study

Two new rat alpha-globin sequences as identified by the conserved region PCR

V M Lam et al. Hemoglobin. 1993 Aug.

Abstract

Based on the notion that regions of structural genes which encode critical domains of the corresponding proteins are highly conserved among closely related species, oligonucleotide primers were designed and used to amplify the alpha-globin sequence(s) of the Sprague-Dawley (SD) rat. Data of these amplified sequence constructs showed that two new rat alpha-globin specific sequences have been identified. Southern hybridization confirmed the presence of these sequences in the rat genome.

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