Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Nov;175(21):6867-72.
doi: 10.1128/jb.175.21.6867-6872.1993.

Oxygen-regulated in vitro transcription of Rhizobium meliloti nifA and fixK genes

J M Reyrat  1 , M David, C Blonski, P Boistard, J Batut
Affiliations

Oxygen-regulated in vitro transcription of Rhizobium meliloti nifA and fixK genes

J M Reyrat et al. J Bacteriol. 1993 Nov.

Abstract

Oxygen concentration regulates the expression of nitrogen fixation genes in the symbiotic bacterium Rhizobium meliloti. We demonstrate that two proteins, FixL and FixJ, that belong to the two-component family of regulatory proteins are necessary and sufficient for oxygen-regulated in vitro transcription of the two key regulatory genes, nifA and fixK. We show directly that FixJ is a transcriptional activator, working in conjunction with the RNA polymerase sigma 70 holoenzyme. Addition of FixL122, a soluble form of the sensor FixL protein, to the transcription assay enhanced FixJ transcriptional activity in response to low oxygen concentration. This enhancement of FixJ activity was correlated with FixJ phosphorylation.

PubMed Disclaimer

References

    1. Nature. 1991 Mar 14;350(6314):170-2 - PubMed
    1. Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3506-10 - PubMed
    1. Mol Microbiol. 1991 Apr;5(4):987-97 - PubMed
    1. Annu Rev Biochem. 1991;60:401-41 - PubMed
    1. J Bacteriol. 1991 Sep;173(18):5914-7 - PubMed

Publication types

MeSH terms

LinkOut - more resources