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. 1993 Nov 15;268(32):23784-7.

Identification of the nerve terminal targets of botulinum neurotoxin serotypes A, D, and E

G Schiavo  1 , O Rossetto, S Catsicas, P Polverino de Laureto, B R DasGupta, F Benfenati, C Montecucco
Affiliations
  • PMID: 8226912
Free article

Identification of the nerve terminal targets of botulinum neurotoxin serotypes A, D, and E

G Schiavo et al. J Biol Chem. .
Free article

Abstract

Botulinum neurotoxins are metalloproteins with one zinc atom bound to the zinc binding motif of zinc endopeptidases. Here we show that botulinum neurotoxin serotypes A, D, and E are zinc endoproteases specific for components of the synaptic vesicle docking and fusion complex. Serotypes A and E cleave SNAP-25, a 25-kDa protein of the synaptic terminal, while serotype D is specific for VAMP/synaptobrevin, a membrane protein of synaptic vesicles. Both rat brain VAMP isoforms are cleaved at a single Lys-Leu peptide bond. The proteolytic activity of these neurotoxins is inhibited by EDTA and captopril.

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