Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1993 Nov 1;90(21):10230-4.
doi: 10.1073/pnas.90.21.10230.

mu opiate receptor: cDNA cloning and expression

J B Wang  1 , Y Imai, C M Eppler, P Gregor, C E Spivak, G R Uhl
Affiliations
Comparative Study

mu opiate receptor: cDNA cloning and expression

J B Wang et al. Proc Natl Acad Sci U S A. .

Abstract

mu opiate receptors recognize morphine with high affinity. A 2.1-kb rat brain cDNA whose predicted translation product displays 63% identity with recently described delta and kappa opiate receptor sequences was identified through polymerase chain reaction and cDNA homology approaches. This cDNA recognizes a 10.5-kb mRNA that is expressed in thalamic neurons. COS-cell expression confers naloxonazine-, Na(+)-, and GTP-sensitive binding of mu but not delta or kappa opioid ligands. Expressing cells bind morphine, [D-Ala2,N-methyl-Phe4,glyol5]enkephalin (DAMGO), and [D-Ala2,D-Leu5]enkephalin (DADLE) with nanomolar or subnanomolar affinities, defining a mu opiate receptor that avidly recognizes analgesic and euphoric opiate drugs and opioid peptides.

PubMed Disclaimer

References

    1. Eur J Pharmacol. 1986 Sep 23;129(1-2):33-8 - PubMed
    1. Pflugers Arch. 1981 Aug;391(2):85-100 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Jun;84(12):4308-12 - PubMed
    1. J Neurosci. 1987 Aug;7(8):2445-64 - PubMed
    1. J Pharmacol Exp Ther. 1987 Jul;242(1):15-20 - PubMed

Publication types

MeSH terms

Associated data