Rapid and simple method for the determination of nitrite in synaptosomal superfusates by liquid chromatography with electrochemical detection

Abstract

A rapid and simple technique using reversed-phase high-performance liquid chromatography with electrochemical detection (HPLC-ED) was developed for the determination of nitrite. Standard solutions of sodium nitrite produced a linear current response over the concentration range 1.5-30 microM. The chromatographic peaks of sodium nitrite were superimposable with peaks obtained after injection into the chromatograph of supernatants from rat cerebellar synaptosomes. The concentrations of nitrite in synaptosomes measured with HPLC-ED were similar to those found with a widely used colorimetric procedure. Therefore, this new method may represent a useful means of measuring nitrite in biological samples.