Effects of glucose, growth temperature, and pH on listeriolysin O production in Listeria monocytogenes
- PMID: 8250571
- PMCID: PMC182482
- DOI: 10.1128/aem.59.10.3495-3497.1993
Effects of glucose, growth temperature, and pH on listeriolysin O production in Listeria monocytogenes
Abstract
Expression of listeriolysin O of Listeria monocytogenes as a function of different growth conditions was studied by performing a direct hemolysin assay, immunoblotting experiments, and an enzyme-linked immunosorbent assay. Expression of listeriolysin O was reduced at a lower growth temperatures (26 degrees C) and at higher glucose concentrations (> or = 0.3%) in the growth media. The effect of glucose appeared to be due to a change in the pH of the growth media.
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