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. 1993 Oct;59(10):3495-7.
doi: 10.1128/aem.59.10.3495-3497.1993.

Effects of glucose, growth temperature, and pH on listeriolysin O production in Listeria monocytogenes

A R Datta  1 , M H Kothary
Affiliations

Effects of glucose, growth temperature, and pH on listeriolysin O production in Listeria monocytogenes

A R Datta et al. Appl Environ Microbiol. 1993 Oct.

Abstract

Expression of listeriolysin O of Listeria monocytogenes as a function of different growth conditions was studied by performing a direct hemolysin assay, immunoblotting experiments, and an enzyme-linked immunosorbent assay. Expression of listeriolysin O was reduced at a lower growth temperatures (26 degrees C) and at higher glucose concentrations (> or = 0.3%) in the growth media. The effect of glucose appeared to be due to a change in the pH of the growth media.

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