[The construction of human genome YAC library and screening by hybridization of membrane high density spotted in array]
[Article in
Chinese]
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- PMID: 8251192
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[The construction of human genome YAC library and screening by hybridization of membrane high density spotted in array]
[Article in
Chinese]
Yi Chuan Xue Bao.
1993.
Abstract
The human genomic YAC (Yeast Artificial Chromosome) library was constructed with human white blood cell and the cell line GM1 414 containing 4 X chromosome DNA using pYAC 4 as vector. Twenty thousand YAC clones were obtained with insert size of 400-1000kb. A set of YACs containing entire DMD geneDNA sequence were selected.
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