Stimulation of cyclic adenosine 3':5'-monophosphate accumulation in human testes in vitro by luteinizing hormone, follicle-stimulating hormone, and prostaglandins

Abstract

The gonadotropins luteinizing hormone (LH) and follicle-stimulating hormone (FSH) stimulate cyclic adenosine 3':5'-monophosphate (cyclic AMP) accumulation in human testicular incubates. The phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine acts synergistically with LH and FSH to enhance cyclic AMP accumulation. These studies indicate that cyclic AMP accumulation may be involved in the mechanism of action of LH and FSH in the human testes, as has been proposed for rat testes. The prostaglandins (PG PGE1, PGE2, PGA1, and PGF2alpha stimulate cyclic AMP levels at 10(-4) M in human testes. The E type prostaglandins are the most potent. They induce half-maximal stimulation of cyclic AMP at 7 x 10(-7) M.

PIP: Luteinizing hormone (LH) and follicle stimulating hormone (FSH) stimulated the accumulation of adenosine 3',5'-monophosphate (cAMP) within 30 minutes of addition to human testicular incubates. The phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine acted synergistically with FSH and to a lesser degree with LH to enhance cAMP accumulation. The findings indicate that cAMP accumulation may be involved in the mechanism of action of LH and FSH in the human testes, as has been proposed for rats. The prostaglandins (PG) PGE-1, PGE-2, PGA-1, and PGF-2-alpha stimulated cAMP levels at a concentration of 1/10,000 M in human testes. The E type prostaglandins were the most potent; they induced half-maximal stimulation of cAMP at 7/10,000,000 M.