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. 1993 Dec;77(4):473-83.
doi: 10.1006/expr.1993.1108.

Plasmodium falciparum: a method for the amplification of S antigens and its application to laboratory and field samples

S Kyes  1 , A G Craig, K Marsh, C I Newbold
Affiliations

Plasmodium falciparum: a method for the amplification of S antigens and its application to laboratory and field samples

S Kyes et al. Exp Parasitol. 1993 Dec.

Abstract

Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplification of S antigen and show why previous attempts may have been unsuccessful. Data are presented from both laboratory and field isolates.

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