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. 1976 Sep 27;283(6):709-11.

[The chromophore of blue dextran: a powerful ligand for the purification of interferon by affinity chromatography]

[Article in French]
J de Maeyer-Guignard, E de Maeyer
  • PMID: 825322

[The chromophore of blue dextran: a powerful ligand for the purification of interferon by affinity chromatography]

[Article in French]
J de Maeyer-Guignard et al. C R Acad Hebd Seances Acad Sci D. .

Abstract

The chromophore of blue dextran can be used as ligand for affinity chromatography of interferon. One passage of a crude interferon preparation on a blue-dextran-sepharose column eliminates over 90% of the contaminating proteins. Desorption with an aqueous buffer of high molarity yields interferon preparations with specific activities of up to 4.5 X 10(6) units per milligram of protein and the recovery is 100%.

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