Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Nov 11;21(22):5110-6.
doi: 10.1093/nar/21.22.5110.

Reconstitution of mammalian excision repair activity with mutant cell-free extracts and XPAC and ERCC1 proteins expressed in Escherichia coli

C H Park  1 , A Sancar
Affiliations
Free PMC article

Reconstitution of mammalian excision repair activity with mutant cell-free extracts and XPAC and ERCC1 proteins expressed in Escherichia coli

C H Park et al. Nucleic Acids Res. .
Free PMC article

Abstract

Nucleotide excision repair in humans involves the coordinated actions of 8-10 proteins. To understand the roles of each of these proteins in excision it is necessary to develop an in vitro excision repair system reconstituted entirely from purified proteins. Towards this goal we have expressed in E. coli two of the 8 genes known to be essential for the excision reaction. XPAC and ERCC1 were expressed as fusion proteins with the Escherichia coli maltose binding protein (MBP) and purified to > 80% homogeneity by affinity chromatography. The purified proteins either as fusions or after cleavage from the MBP were able to complement the CFE of cells with mutations in the corresponding genes in an excision assay with thymine dimer containing substrate.

PubMed Disclaimer

References

    1. J Biol Chem. 1992 Aug 15;267(23):16601-6 - PubMed
    1. Nucleic Acids Res. 1992 Jul 11;20(13):2441-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8273-7 - PubMed
    1. J Biol Chem. 1993 Jan 25;268(3):1931-6 - PubMed
    1. J Biol Chem. 1993 Apr 25;268(12):9105-9 - PubMed

Publication types

MeSH terms