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. 1976 Nov;73(11):4145-9.
doi: 10.1073/pnas.73.11.4145.

Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli

S D Ehrlich, H Bursztyn-Pettegrew, I Stroynowski, J Lederberg

Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli

S D Ehrlich et al. Proc Natl Acad Sci U S A. 1976 Nov.

Abstract

The thymidylate synthetase gene of B. subtilis bacteriophage Phi-3-T, when cloned in plasmids pSC101 or pMB9 is expressed in E. coli. The promoter of the cloned gene is likely to originate in Phi-3-T. Rearrangements of hybrid plasmid sequences during the cloning have been noted. B. subtilis strains can be transformed with hybrid DNAs. The transformants contain sequences of Phi-3-T, but not those of plasmid vectors.

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References

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