Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978;24(3-4):305-9.
doi: 10.1016/0022-1759(78)90133-3.

A rapid, novel method for the solid-phase derivatization of IgG antibodies for immune-affinity chromatography

D M Gersten, J J Marchalonis

A rapid, novel method for the solid-phase derivatization of IgG antibodies for immune-affinity chromatography

D M Gersten et al. J Immunol Methods. 1978.

Abstract

We show that a solid-phase immune adsorbent can be prepared from rabbit antiserum by isolating the IgG fraction with the A protein of Staphylococcus aureus associated covalently with a Sepharose matrix. The IgG is then coupled to the matrix using the cross-linking agent dimethylsuberimidate. IgG antibody bound in this fashion is in the proper orientation for combination with antigen because association with protein A occurs via the Fc portion of the IgG molecule, thus leaving the combining site of the molecule free to interact with antigen.

PubMed Disclaimer

Publication types

LinkOut - more resources