doi: 10.1016/0022-1759(78)90133-3.
A rapid, novel method for the solid-phase derivatization of IgG antibodies for immune-affinity chromatography
- PMID: 82592
- DOI: 10.1016/0022-1759(78)90133-3
Item in Clipboard
A rapid, novel method for the solid-phase derivatization of IgG antibodies for immune-affinity chromatography
J Immunol Methods.
1978.
Abstract
We show that a solid-phase immune adsorbent can be prepared from rabbit antiserum by isolating the IgG fraction with the A protein of Staphylococcus aureus associated covalently with a Sepharose matrix. The IgG is then coupled to the matrix using the cross-linking agent dimethylsuberimidate. IgG antibody bound in this fashion is in the proper orientation for combination with antigen because association with protein A occurs via the Fc portion of the IgG molecule, thus leaving the combining site of the molecule free to interact with antigen.
Similar articles
-
Immunochemical studies to purify rabbit and chicken immunoglobulin G antibody by protein A-Sepharose chromatography.Am J Vet Res. 1983 May;44(5):901-6. Am J Vet Res. 1983. PMID: 6408954
-
Anti-idiotypic antibody mimicking a T-antigen-specific lectin inhibits human epithelial tumor cell proliferation.Immunol Cell Biol. 2010 Nov-Dec;88(8):787-94. doi: 10.1038/icb.2010.49. Epub 2010 Apr 20. Immunol Cell Biol. 2010. PMID: 20404839
-
Purification of immunoreactive radiolabeled monoclonal antibodies with anti-idiotypic monoclonal antibodies.J Immunol Methods. 1990 Feb 9;126(2):223-9. doi: 10.1016/0022-1759(90)90154-n. J Immunol Methods. 1990. PMID: 2406347
-
The solid phase in affinity chromatography: strategies for antibody attachment.J Biochem Biophys Methods. 2001 Oct 30;49(1-3):467-80. doi: 10.1016/s0165-022x(01)00214-7. J Biochem Biophys Methods. 2001. PMID: 11694295 Review.
-
Use of staphylococcal protein A as an immunological reagent.J Immunol Methods. 1978;20:241-53. doi: 10.1016/0022-1759(78)90259-4. J Immunol Methods. 1978. PMID: 349081 Review.
Cited by
-
Identification of sulfated oligosialic acid units in the O-linked glycan of the sea urchin egg receptor for sperm.Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):3650-5. doi: 10.1073/pnas.94.8.3650. Proc Natl Acad Sci U S A. 1997. PMID: 9108032 Free PMC article.
-
Linkage of 17 beta-oestradiol dehydrogenase to actin by epsilon-(gamma-glutamyl)-lysine in porcine endometrial cells.Biochem J. 1993 Dec 15;296 ( Pt 3)(Pt 3):797-802. doi: 10.1042/bj2960797. Biochem J. 1993. PMID: 8280079 Free PMC article.
-
Oriented covalent immobilization of antibodies for measurement of intermolecular binding forces between zipper-like contact surfaces of split inteins.Anal Chem. 2013 Jun 18;85(12):6080-8. doi: 10.1021/ac400949t. Epub 2013 May 31. Anal Chem. 2013. PMID: 23679912 Free PMC article.
-
Novel biochemical, structural, and systems insights into inflammatory signaling revealed by contextual interaction proteomics.Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2117175119. doi: 10.1073/pnas.2117175119. Epub 2022 Sep 30. Proc Natl Acad Sci U S A. 2022. PMID: 36179048 Free PMC article.
-
Direct cross-linking of snRNP proteins F and 70K to snRNAs by ultra-violet radiation in situ.Nucleic Acids Res. 1988 Dec 9;16(23):10985-1004. doi: 10.1093/nar/16.23.10985. Nucleic Acids Res. 1988. PMID: 2974540 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
