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. 1993 Nov;31(11):3028-30.
doi: 10.1128/jcm.31.11.3028-3030.1993.

Sensitive identification of bluetongue virus serogroup by a colorimetric dual oligonucleotide sorbent assay of amplified viral nucleic acid

J B Katz  1 , A D Alstad, G A Gustafson, K M Moser
Affiliations

Sensitive identification of bluetongue virus serogroup by a colorimetric dual oligonucleotide sorbent assay of amplified viral nucleic acid

J B Katz et al. J Clin Microbiol. 1993 Nov.

Abstract

A polymerase chain reaction (PCR) procedure coupled to an enzyme-linked oligonucleotide sorbent assay (ELOSA; a PCR-ELOSA) identified all 24 serotypic variants of bluetongue virus (BTV) without identifying any of six viruses belonging to the related epizootic hemorrhagic disease virus serogroup. The PCR-ELOSA detected 0.01 50% cell culture infectious doses of each serotype of BTV. The sensitivity and serogroup-wide specificity of the PCR-ELOSA may enable it to replace the more expensive, time-consuming, and biohazardous methods used in the identification of BTV.

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References

    1. J Clin Microbiol. 1992 Apr;30(4):845-53 - PubMed
    1. J Immunol Methods. 1992 Jun 24;150(1-2):33-49 - PubMed
    1. Mol Cell Probes. 1992 Jun;6(3):245-9 - PubMed
    1. J Vet Diagn Invest. 1992 Oct;4(4):400-5 - PubMed
    1. J Virol Methods. 1991 May;32(2-3):171-80 - PubMed

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