[Detection of herpes simplex virus 1 and 2 (HSV-1 and HSV-2) IgG and IgM by ELISA in cord blood and sera of mothers with pregnancy complications]

Abstract

In this study, HSV-1 IgG, IgM and HSV-2 IgG, IgM antibodies by ELISA in the sera of the mothers who had different kinds of obstetrical problems like abortus, stillbirth, prematurity, postmaturity, intrauterine development retardation and in the newborns' cord sera who had congenital anomalies like anencephaly, cataract and dolichocephaly were investigated. In these mothers HSV-1 IgG positivity ratio was 71/73 (97.3%). There was no significant difference in the age group distribution of HSV-1 IgG. The HSV-1 IgG positivity ratios in mother sera were the same as in cord sera. It was found that HSV-1 IgG antibodies passed transplacentally. It was established that one of the subjects had premature baby and the other had stillbirth inspite of HSV-1 IgG negativity but in the risk group subjects. HSV-1 IgM was found positive in 7 mothers (9.6%) of the study group. This data was the sign of active or reactive infection. There were 3 abortus, 1 stillbirth and 3 anomalous baby in HSV-1 IgM seropositive mothers. In these mothers, 65/73 (89%) HSV-2 IgG and 6/73 (8%) HSV-2 IgM seropositivity were defined. In the cord sera these ratios were 65/73 (89%) and 2/73 (2.7%). IgM seropositivity of the material of the mothers who had abortus was explained by the effect of HSV-2 activity and the presence of HSV-2 IgM, 4 mothers were noted for the active or reactive infection sign. It was understood that HSV-2 IgG passed completely via placenta and there were no significant difference between the presence of the antibody and the age group.