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. 1993 Dec;241(5-6):497-503.
doi: 10.1007/BF00279891.

Cloning, sequencing and enhanced expression of the Trichoderma reesei endoxylanase II (pI 9) gene xln2

R Saarelainen  1 , M Paloheimo, R Fagerström, P L Suominen, K M Nevalainen
Affiliations

Cloning, sequencing and enhanced expression of the Trichoderma reesei endoxylanase II (pI 9) gene xln2

R Saarelainen et al. Mol Gen Genet. 1993 Dec.

Abstract

The Trichoderma reesei xln2 gene coding for the pI9.0 endoxylanase was isolated from the wild-type strain QM6a. The gene contains one intron of 108 nucleotides and codes for a protein of 223 amino acids in which two putative N-glycosylation target sites were found. Three different T. reesei strains were transformed by targeting a construct composed of the xln2 gene, including its promoter, to the endogenous cbh1 locus. Highest overall production levels of xylanase were obtained using T. reesei ALKO2721, a genetically engineered strain, as a host. Integration into the cbh1 locus was not required for enhanced expression under control of the xln2 promoter.

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References

    1. Enzyme Microb Technol. 1991 Mar;13(3):227-33 - PubMed
    1. J Mol Biol. 1983 Jun 5;166(4):557-80 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1470-4 - PubMed
    1. Gene. 1987;51(1):43-52 - PubMed
    1. EMBO J. 1985 Feb;4(2):475-9 - PubMed

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