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Comparative Study
. 1993;40(4):241-5.

HPLC analysis of optical isomers of leucovorin and methotrexate using achiral-chiral system

E Brandsteterová  1 , K Marcincinová, J Lehotay, A Zbojová, J Halko
Affiliations
  • PMID: 8272151
Comparative Study

HPLC analysis of optical isomers of leucovorin and methotrexate using achiral-chiral system

E Brandsteterová et al. Neoplasma. 1993.

Abstract

Chiral high performance liquid chromatography was used for the enantiometric separation of leucovorin. The optimal separation conditions recommended after optimizing the mobile phase composition, flow rate, and temperature are described. Achiral reversed-phase chromatographic method was applied for the simultaneous separation of methotrexate and leucovorin in clinical samples. Column-switching system including achiral short pre-column and chiral analytical column based on immobilized bovine serum albumin were used for simultaneous determination of leucovorin and methotrexate patients treated at the National Cancer Institute.

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