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. 1976 Dec;9(4 Pt 1):551-7.
doi: 10.1016/0092-8674(76)90037-4.

The blue-green alga agmenellum quadruplicatum contains covalently closed DNA circles

T M Roberts, K E Koths

The blue-green alga agmenellum quadruplicatum contains covalently closed DNA circles

T M Roberts et al. Cell. 1976 Dec.

Abstract

This paper reports the first direct visualization of covalently closed, circular DNA from a blue-green alga. Agmenellum quadruplicatum lysates, fractionated on CsCl-ethidium bromide density gradients, yield a minor band of covalently closed DNA circles which we have analyzed by electron microscopy and agarose gel electrophoresis. The DNA consists of a number of a discrete classes of DNA circles ranging in molecular weight from 3 X 10(6) daltons to 65-80 X 10(6) daltons. The function of these circles is at present entirely unknown.

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