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. 1976 Nov 2;110(23):145-7.
doi: 10.1007/BF00690221.

Heterotrophic micro- and macrocultures of a nitrogen-fixing cyanobacterium

C P Wolk, P W Shaffer

Heterotrophic micro- and macrocultures of a nitrogen-fixing cyanobacterium

C P Wolk et al. Arch Microbiol. .

Abstract

Anabaena variabilis can be cloned in the dark from fragments with one and few cells, with an efficiency of about 40%, on the nitrogen-free medium of Allen and Arnon solidified with 0.5% agarose and supplemented with 5 mM fructose. The organism can be grown exponentially (tau2 approximately or equal to 36 h) in fermentor cultures in the dark, fixing N2, to a density of greater than 10 g dry weight/l.

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