Effects of nightbreak, T-cycle, and resonance lighting schedules on the pineal melatonin rhythm of the lizard Anolis carolinensis: correlations with the reproductive response

Abstract

Experiments were conducted to determine if a correlation exists between any aspect of the pineal melatonin rhythm (such as its duration or phase) in the lizard Anolis carolinensis and the reproductive response to photoperiod. The rhythm of pineal melatonin content was determined in anoles exposed to nightbreak lighting protocols (10L:5D:1L:8D, 10L:10D:1L:3D), resonance lighting cycles (LD 11:13, LD 11:25), and T-cycle lighting protocols (LD 11:7, LD 11:9, LD 11:13, LD 11:15, LD 11:19) and compared with the testicular response to these lighting protocols as determined previously [Underwood and Hyde, (1990) J. Comp. Physiol. (A) 167:231-243]. Different T-cycles and nightbreak cycles elicited changes in both the duration of the melatonin peak and the phase of the melatonin peak relative to these light cycles. The response to the resonance cycle (LD 11:25) was complex, probably due to the overlapping patterns of two groups whose pineal melatonin rhythms were entrained approximately 12 hr out of phase with each other. No correlation was observed between the duration, or the amplitude, of the nocturnal melatonin peaks seen on the various light cycles and the reproductive response to these cycles. A correlation was observed between the phase of the pineal melatonin rhythm and the reproductive response. Light cycles were inductive (stimulated testicular growth) when the entrained melatonin rhythm peaked near the light-to-dark or the dark-to-light transition, but they were not inductive when the melatonin rhythm peaked during the middle third of the night. These results suggest that if melatonin is involved in the transduction of photoperiodic information in Anolis, neither the duration nor amplitude of the nocturnal melatonin pulse is involved in the measurement of day length. Instead, the phase-relationship of the melatonin rhythm to the rest of the circadian system may determine photoperiodic responsiveness.