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. 1994 Jan;13(1):83-6.
doi: 10.1089/dna.1994.13.83.

An efficient method for large-scale isolation of plasmid DNAs by heat-alkali co-denaturation

N E Sun  1 , B H Shen, J M Zhou, J Yuan, X X Xu, D X Zhu, K K Han
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An efficient method for large-scale isolation of plasmid DNAs by heat-alkali co-denaturation

N E Sun et al. DNA Cell Biol. 1994 Jan.

Abstract

A method is presented for efficient and large-scale isolation of plasmid DNAs from bacterial cells. Based on the cooperativity of heat and alkali actions, the method provides DNA preparations with high quality and yield (about 2 micrograms of DNA/ml culture), which are completely digestable by restriction enzymes and have a high transformation efficiency. Furthermore, the DNA preparations are extremely stable, and even through 4-year storage at -20 degrees C, the electrophorogram and transformation efficiency remain as high as before. The factors affecting the stability of various DNA samples are discussed.

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