Mutations in the delta subunit influence the assembly of F1F0 ATP synthase in Escherichia coli
- PMID: 8288552
- PMCID: PMC205083
- DOI: 10.1128/jb.176.2.540-542.1994
Mutations in the delta subunit influence the assembly of F1F0 ATP synthase in Escherichia coli
Abstract
Missense mutations affecting Asp-161 and Ser-163 in the delta subunit of F1F0 ATP synthase have been generated. Although most substitutions allowed substantial enzyme function, the delta Asp-161-->Pro substitution resulted in a loss of enzyme activity. The loss of activity was attributable to a structural failure altering assembly of the enzyme complex.
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