Histomorphometric study of dental pulp during orthodontic tooth movement

Abstract

Orthodontic tooth movement has been implicated in secondary changes to the dental pulp. The purpose of this study was to correlate the effects of orthodontic tooth movement on the dental pulp by histomorphometric parameters. Four groups, each consisting of 36 male adult Sprague-Dawley strain rats, were studied with differing force magnitudes. These included a sham group in addition to groups with bilaterally placed appliances activated to 20, 40, and 60 g of initial force designed to mesially tip the maxillary first molars. Six rats were killed at 1, 3, 5, 7, 10, and 14 days. Specimens were fixed, embedded, and stained with tetrachrome. Pulpal measurements were made with an image analyzer and included changes in predentin and vascularity. Findings indicated a significant increase (p < or = 0.05) relative to time and force magnitude in capillary number. An initial pulpal hyperemia was observed following activation of orthodontic force which was unrelated to force magnitude. A force-dependent increase in predentin width was measured at the peak of the tooth movement cycle.