[Separation of the hormones of the adenohypophysis of rats by use of electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulfate]

Abstract

A comparative study of rat adenohypophysis extract and its alcohol fractions was performed by two variants of the method of electrophoresis in polyacrylamide gel: at pH 9,5 and with the presence of sodium dodecyl sulphate at pH 7.2. With the presence of sodium dodecylsulphate four protein zones are found which in the order from the anode towards the cathode are identified as hemoglobin, somatotropin, lactotropin and albumin. It is shown that the somatotropin zone after the extract separation at pH 9.5 is inhomogeneous and consists of somatotropin and hemoglobin.