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. 1993 Dec 25;21(25):5843-5.
doi: 10.1093/nar/21.25.5843.

Differences in the accessibility of methylated and unmethylated DNA to DNase I

S Kochanek  1 , D Renz, W Doerfler
Affiliations
Free PMC article

Differences in the accessibility of methylated and unmethylated DNA to DNase I

S Kochanek et al. Nucleic Acids Res. .
Free PMC article

Abstract

DNase I binds in the minor groove of DNA and is used as an enzymatic tool to investigate the interaction of proteins with DNA. Here we show that the major groove located 5-methyldeoxycytidine can enhance or inhibit the cleavage rates of DNA by DNase I. This effect may be caused in part by changes in DNA structure affecting the accessibility of the minor groove of DNA to DNase I.

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