doi: 10.1093/nar/5.11.4055.
Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae
- PMID: 82935
- PMCID: PMC342733
- DOI: 10.1093/nar/5.11.4055
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Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae
Nucleic Acids Res.
1978 Nov.
Free PMC article
Abstract
We have determined the recognition sequence of the restriction endonuclease KpnI, previously isolated from Klebsiella pneumoniae. The enzyme cleaves the twofold rotationally symmetric sequence (see book for formula) at the positions indicated by the arrows, producing 3' protruding cohesive ends, four nucleotides in length. The specific cleavage site was unambiguously deduced using both 3' and 5' end analyses of KpnI generated restriction fragments of simian-virus 40 (SV40) DNA (1 site), adenovirus-2 (Ad-2) DNA (8 sites), and a plasmid (pCRI) DNA (2 sites).
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