Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Nov;5(11):4055-64.
doi: 10.1093/nar/5.11.4055.

Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae

J Tomassini, R Roychoudhury, R Wu, R J Roberts
Free PMC article

Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae

J Tomassini et al. Nucleic Acids Res. 1978 Nov.
Free PMC article

Abstract

We have determined the recognition sequence of the restriction endonuclease KpnI, previously isolated from Klebsiella pneumoniae. The enzyme cleaves the twofold rotationally symmetric sequence (see book for formula) at the positions indicated by the arrows, producing 3' protruding cohesive ends, four nucleotides in length. The specific cleavage site was unambiguously deduced using both 3' and 5' end analyses of KpnI generated restriction fragments of simian-virus 40 (SV40) DNA (1 site), adenovirus-2 (Ad-2) DNA (8 sites), and a plasmid (pCRI) DNA (2 sites).

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1974 Mar;1(3):331-53 - PubMed
    1. J Biol Chem. 1964 Aug;239:2628-36 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Mar;74(3):966-70 - PubMed
    1. Nature. 1977 Dec 8;270(5637):486-94 - PubMed
    1. Cell. 1977 Mar;10(3):509-19 - PubMed

Publication types