Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Dec 22;136(1-2):287-90.
doi: 10.1016/0378-1119(93)90481-h.

A PCR method for the sequence analysis of the gyrA, polA and rnhA gene segments from mycobacteria

V Mizrahi  1 , P Huberts, S S Dawes, L R Dudding
Affiliations

A PCR method for the sequence analysis of the gyrA, polA and rnhA gene segments from mycobacteria

V Mizrahi et al. Gene. .

Abstract

Internal segments of the gyrA and polA genes involved in DNA replication of Mycobacterium tuberculosis and rnhA of M. smegmatis, have been amplified by the polymerase chain reaction (PCR) using degenerate oligodeoxyribonucleotide primers based on conserved sequences. The deduced amino acid sequences were 54-66% homologous to the corresponding segments of their Escherichia coli counterparts. This method provides a useful means of cloning genes encoding DNA replication enzymes of mycobacteria.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources