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Comparative Study
. 1993;79(8):699-702.
doi: 10.1007/BF00932514.

High-performance liquid chromatographic analysis of monoamines in the cestode Diphyllobothrium dendriticum

K Eriksson  1 , M Gustafsson, G Akerlind
Affiliations
Comparative Study

High-performance liquid chromatographic analysis of monoamines in the cestode Diphyllobothrium dendriticum

K Eriksson et al. Parasitol Res. 1993.

Abstract

The presence of biogene monoamines in adult and larval Diphyllobothrium dendriticum (Cestoda) was investigated by high-performance liquid chromatography with electrochemical detection (HPLC-ED). The biogene amines serotonin (5-HT), dopamine (DA), noradrenaline (NA), and adrenaline (A) as well as many of their precursors and metabolites, comprising a total of 15 different substances, were analyzed. 5-HT, DOPA, DA, NA, and A were detected in the worm, with 5-HT, DOPA, and DA being the dominating amines. The DA metabolites DOPAC and 3-MT or the 5-HT precursor 5-hydroxytryptophan could not be detected, but two unidentified substances, believed to be catecholic, were present in the worm. A high concentration of DOPA was measured in the proglottids and especially in the eggs. This is the first report of A in a flatworm.

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