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. 1993 Nov-Dec;7(6):315-6.
doi: 10.1002/bmc.1130070607.

Thin-layer chromatographic determination of brain catecholamines and 5-hydroxytryptamine

G Alemany  1 , M C Nicolau, A Gamundí, R Rial
Affiliations

Thin-layer chromatographic determination of brain catecholamines and 5-hydroxytryptamine

G Alemany et al. Biomed Chromatogr. 1993 Nov-Dec.

Abstract

A simple and sensitive (up to nanogram level) method to determine norepinephrine, serotonin and dopamine in rat brain is described. The amines are acetylated and the derivatives are resolved by thin layer chromatography (TLC) on silica high performance thin layer chromatography (HPLC) plates. Quantification is achieved by fluorescence densitometry at 415 nm excitation wavelength.

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