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. 1993 Dec;24(6):515-9.
doi: 10.1007/BF00351715.

Isolation, characterization and mapping of pyrimidine auxotrophs of Phycomyces blakesleeanus

V Campuzano  1 , P del Valle, J I de Vicente, A P Eslava, M I Alvarez
Affiliations

Isolation, characterization and mapping of pyrimidine auxotrophs of Phycomyces blakesleeanus

V Campuzano et al. Curr Genet. 1993 Dec.

Abstract

A total of seven pyrimidine auxotrophs of Phycomyces were isolated from among 5-fluoroorotate acid (5-FOA)-resistant mutants. They were classified by complementation into two groups. A representative mutant strain belonging to one group was deficient in orotate phosphoribosyl transferase (OPRTase; EC 2.4.2.10) activity; the mutant strain belonging to the second group was deficient in orotidine-5'-monophosphate decarboxylase (OMPdecase; EC 4.1.1.23). These mutants are defective in the genes pyrF and pyrG respectively. The results from random spore analysis, tetrad analysis, and gene-centromere distances showed that these two markers are located in linkage group VI, with pyrG being a proximal marker and pyrF a distal one.

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