Identification of alternate 5' untranslated regions in the gene encoding Ca2+/calmodulin-dependent kinase-G1

Abstract

Alternate 5' untranslated regions (UTR) of the Ca2+/calmodulin-dependent kinase-Gr cDNA were identified by sequencing of anchored reverse transcription-polymerase chain reaction (RT-PCR) products. Three alternate 5' ends of the mRNA were spliced at the same point to a common 5' UTR. A 3-kb EcoRI-BamHI genomic DNA restriction fragment containing the alternate UTR and 5' flanking sequence was characterized by sequence analysis and identification of several potential transcriptional control elements.