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. 1993 Nov;30(3):312-5.
doi: 10.1006/plas.1993.1066.

A useful cloning vector for Bacillus subtilis

B Diderichsen  1 , G B Poulsen, S T Jørgensen
Affiliations

A useful cloning vector for Bacillus subtilis

B Diderichsen et al. Plasmid. 1993 Nov.

Abstract

We have constructed plasmid pDN1050, a new small cloning vector for Bacillus subtilis. pDN1050 harbors the origin of replication of Staphylococcus aureus plasmid pUB110 and the chloramphenicol resistance gene of S. aureus plasmid pC194. The plasmid is segregationally and structurally stable. Plasmid pDN1370, a low copy number mutant of pDN1050 was isolated and shown to harbor a mutation in the repA gene of the replication protein.

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