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. 1978 Oct 30;92(3):309-13.
doi: 10.1007/BF00461654.

Bleomycin: effect on satellite DNA in mouse fibroblasts

H Madreiter, R Osieka, C Wittekind, P Kaden, C Mittermayer

Bleomycin: effect on satellite DNA in mouse fibroblasts

H Madreiter et al. Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. .

Abstract

The effect of Bleomycin on the semiconservative replication of mouse nuclear DNA has been studied. When asynchronously dividing mouse fibroblasts (L-cells) were grown in the presence of 5-bromodeoxyuridine (25 mg/l medium) for 18 h, three hybrid DNA bands with densities of 1.722, 1.752, and 1.761 kg/l appeared after caesium chloride density gradient centrifugation of nuclear DNA. In cells exposed to Bleomycin (100 mg/l) however, replication of satellite DNA is more strongly inhibited than is the replication of the main band DNA; preferentially the thymidinerich hybrid duplex at 1.761 kg/l could no longer be detected.

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