A 'zebra' chromosome arising from multiple translocations involving non-homologous chromosomes

Abstract

An alloplasmic wheat line carrying a 'zebra' chromosome z5A was isolated from the derivatives of an Elymus trachycaulus x Triticum aestivum cv Chinese Spring hybrid. Chromosome z5A was named zebra because of its striped genomic in situ hybridization pattern. z5A consists of four chromosome segments derived from E. trachycaulus and four chromosome segments, including the centromere, from wheat. The short arm of z5A paired with the telocentric chromosome 1H(t)S of E. trachycaulus and the long arm with the long arm of normal 5A. z5A also carried several genetic markers derived from 1H(t)S. Chromosome 1H(t) was the only E. trachycaulus chromosome found in the sib plants of a previous generation from which z5A was derived. Monosomic 5A and telocentric chromosome 5AL were also found in most of the sib plants. The zebra chromosome most probably originated from spontaneous multiple translocations between chromosomes 5A and 1H(t)S or 5A and 1H(t).