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. 1993 Dec;31(12):3344-7.
doi: 10.1128/jcm.31.12.3344-3347.1993.

New method for detection of Candida albicans in human blood by polymerase chain reaction

Y Miyakawa  1 , T Mabuchi, Y Fukazawa
Affiliations

New method for detection of Candida albicans in human blood by polymerase chain reaction

Y Miyakawa et al. J Clin Microbiol. 1993 Dec.

Abstract

We developed a new method for detection of Candida albicans cells in human blood by the polymerase chain reaction that amplifies a 125-bp region within a species-specific, multiple-copy DNA fragment. In preparing template DNA from C. albicans-containing blood, the addition of C. tropicalis cells, together with antibody which reacts with both cells, raised the sensitivity of detection of C. albicans to about 3 cells per 0.1 ml.

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