Different sites of polyadenylation in mRNAs encoding a rat metabotropic glutamate receptor

Abstract

Two new complementary DNAs overlapping cDNA clones that encode the G-protein coupled metabotropic glutamate receptor mGluR1 from rat brain have been isolated and sequenced in their entirety. These new clones represent mRNA with 3' untranslated regions approximately 2.5 kilobases longer than the previously isolated cDNA clones. These results indicate that the previously observed two size classes of approximately 4 kb and approximately 7 kb which hybridize to sequences that encode this receptor use different polyadenylation signals and differ in the extent of their 3' untranslated sequence. There is a striking asymmetry in the distribution of a sequence involved in mRNA instability between the two mRNA species.