Immunoreactive proteins of Acanthocheilonema viteae (Nematoda: Filarioidea) adults: solubilization in various detergents

Abstract

For optimal solubilization of immunoreactive proteins of filarial parasites, adult worms of Acanthocheilonema viteae were extracted in different detergents including anionic, cationic, nonionic and zwitterionic agents under varying incubation periods. Each preparation was then analysed by SDS-PAGE and immunoblotting using pooled sera from Mastomys natalensis infected with A. viteae. Amongst the detergents used, maximum immunoreactive proteins were exposed by sodium dodecylsulphate (SDS), closely followed by sodium deoxycholate (DOC). Nevertheless, a few additional protein bands were recognized by infected sera in DOC preparations, but not in SDS, and vice versa. Most of the proteins were completely or partially dissolved within 2 hrs extraction time. It is felt that DOC may be used in place of SDS because of the strong denaturing character of the latter.