doi: 10.1016/0005-2787(77)90221-0.
Analysis of rabbit brain polysomal poly (A+) mRNA by DNA excess hybridization
- PMID: 831808
- DOI: 10.1016/0005-2787(77)90221-0
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Analysis of rabbit brain polysomal poly (A+) mRNA by DNA excess hybridization
Biochim Biophys Acta.
.
Abstract
DNA sequence representation in rabbit brain mRNA was examined by DNA excess hybridization. purified polysomal poly (A+) mRNA was labeled in vitro with [3H] dimethyl sulfate and reacted at DNA: RNA ratios of 5000 : 1 and 30 000 : 1. Poly(A+) mRNA hybridizied mainly to nonrepeated DNA with a smaller kinetic component which was complementary to repeated DNA sequences. The latter component was not due to nuclear or ribosomal RNA contamination. Poly(A-) RNA purified from brain polysomes hybridized to excess DNA as a single repeated component. Thermal denaturation profiles of the RNA - DNA hybrids indicated a high degree of fidelity in base pairing.
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