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. 1993 May-Jun;23(5-6):542-6.
doi: 10.1007/BF00312649.

Gene replacement and ectopic integration in the zygomycete Mucor circinelloides

J Arnau  1 , P Strøman
Affiliations

Gene replacement and ectopic integration in the zygomycete Mucor circinelloides

J Arnau et al. Curr Genet. 1993 May-Jun.

Abstract

Transformation of Mucor circinelloides with plasmid pTL42 led to the identification of different integrative events. Analysis of eight transformed strains allowed the detection of three classes of transformants: integrative types derived from either homologous (exclusively at the leuA locus) or heterologous recombination and a class which originated by gene replacement. No autonomous replication of the vector was observed.

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